By Nancy Clanton, The Atlanta Journal-Constitution

Troy Warren for CNT

Researchers suggest saccharin, sucralose and aspartame can cause healthy gut bacteria to become diseased and invade the gut wall

Numerous studies have been done on artificial sweeteners, with some showing they can alter the number and type of bacteria in the gut. A new study, however, led by academics from Anglia Ruskin University, has demonstrated that sweeteners can also make the bacteria pathogenic, or causing disease.

The researchers suggest common artificial sweeteners can cause previously healthy gut bacteria to become diseased and invade the gut wall, potentially leading to serious health issues.

The study, according to the research team, is the first to show the pathogenic effects of saccharin, sucralose and aspartame on two types of gut bacteria: E. coli (Escherichia coli) and E. faecalis (Enterococcus faecalis). It found these pathogenic bacteria can attach themselves to, invade and kill Caco-2 cells, which are epithelial cells that line the wall of the intestine.

According to the study’s results, at a concentration equal to two cans of diet soda, all three artificial sweeteners significantly increased the adhesion of both E. coli and E. faecalis to intestinal Caco-2 cells, and differentially increased the formation of biofilms.

Bacteria growing in biofilms are less sensitive to antimicrobial resistance treatment and are more likely to secrete toxins and express virulence factors, which are molecules that can cause disease.

In addition, all three sweeteners caused the pathogenic gut bacteria to invade Caco-2 cells found in the wall of the intestine, with the exception of saccharin, which had no significant effect on E. coli invasion.

Senior author Dr. Havovi Chichger, senior lecturer in biomedical science at Anglia Ruskin, said in a release by the university: “There is a lot of concern about the consumption of artificial sweeteners, with some studies showing that sweeteners can affect the layer of bacteria which support the gut, known as the gut microbiota.

“Our study is the first to show that some of the sweeteners most commonly found in food and drink — saccharin, sucralose and aspartame — can make normal and ‘healthy’ gut bacteria become pathogenic. These pathogenic changes include greater formation of biofilms and increased adhesion and invasion of bacteria into human gut cells.

“These changes could lead to our own gut bacteria invading and causing damage to our intestine, which can be linked to infection, sepsis and multiple-organ failure.

“We know that overconsumption of sugar is a major factor in the development of conditions such as obesity and diabetes. Therefore, it is important that we increase our knowledge of sweeteners versus sugars in the diet to better understand the impact on our health.”

The study was published in the International Journal of Molecular Sciences.